Condition | Retention time | Number of theoretical plates | USP Tailing factor | Resolution | Capacity factor (k) (CNB) | |||
---|---|---|---|---|---|---|---|---|
CNB | H-impurity | CNB | H-impurity | CNB | H-impurity | |||
Optimum condition | ||||||||
Thermo, Hypersil BDS C18 column (150 × 4.6 mm, 5 μm), mobile phase: Methanol: purified water 50:50, %v/v | 3.2 | 4.14 | 7591 | 8369 | 1.06 | 1.04 | 5.8 | 2.56 |