Table 5 System suitability parameters for the developed RP-HPLC-PDA method
Parameters | Imipenem | Ertapenem | Cilastatin | Meropenem | Probenecid | Warfarin | Reference value | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Retention time (tR) (min) | 3.83 | 11.67 | 12.88 | 19.59 | 21.13 | 21.67 | –– | ||||
Capacity factor (K’) | 2.83 | 10.67 | 11.88 | 18.95 | 20.13 | 20.67 | > 2 | ||||
Tailing factor (Tf) | 1.0 | 0.77 | 0.75 | 1.0 | 1.0 | 1.0 | Less than 2 | ||||
Asymmetry Factor (Af) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 0.9–1.5 | ||||
Resolution (Rs) | 13.06 | 1.86 | 12.20 | 3.42 | 1.80 | ≥ 1.5 | |||||
Column efficiency (N) | 2704 | > 2000 | |||||||||