Table 4 Design expert generated runs according to selected factors
Run | Factor 1 | Factor 2 | Factor 3 |
|---|---|---|---|
A:% organic phase | B:pH Buffer | C:Flow Rate | |
1 | 40.0 | 5.0 | 0.3 |
3 | 50.0 | 5.0 | 0.3 |
4 | 50.0 | 5.0 | 0.2 |
6 | 50.0 | 5.0 | 0.3 |
8 | 50.0 | 5.0 | 0.3 |
10 | 50.0 | 5.0 | 0.3 |
11 | 50.0 | 5.0 | 0.3 |
12 | 50.0 | 5.0 | 0.4 |
15 | 50.0 | 5.0 | 0.3 |
7 | 60.0 | 5.0 | 0.3 |
2 | 40.0 | 3.0 | 0.2 |
5 | 40.0 | 3.0 | 0.4 |
17 | 50.0 | 3.0 | 0.3 |
9 | 60.0 | 3.0 | 0.4 |
14 | 60.0 | 3.0 | 0.2 |
18 | 40.0 | 7.0 | 0.2 |
20 | 40.0 | 7.0 | 0.4 |
16 | 50.0 | 7.0 | 0.3 |
13 | 60.0 | 7.0 | 0.2 |
19 | 60.0 | 7.0 | 0.4 |