Fig. 4

Effects of DLP-1 and DLP-2 on mRNA and protein expression of TLR-4, MyD88 and TRAF-6. Cells were treated with LPS (1 μg/mL) for 24 h in the presence or absence of A DLP-1 (5 μg/mL) and B DLP-2 (50 μg/mL), respectively. The mRNA expression levels were determined by qRT-PCR. The protein expression was detected by western blotting assay. β-Actin was used as loading control. Values were mean ± SD (n = 3), bars with the same letter were not significantly different at P < 0.05, in accordance with Duncan’s multiple range test