Optimized method for determination of 16 FDA polycyclic aromatic hydrocarbons (PAHs) in mainstream cigarette smoke by gas chromatography–mass spectrometry

Table 5 PAH levels in 3R4F ISO MCS obtained by three GC/MS systems using ¹³C-labelled internal standards

PAH	GC–HRMS		GC–MS/MS		GC–MS		IS recovery^a		Published values, (ng/cig) [10]	Published values, (ng/cig) [13]	Published values, (ng/cig) [37]
PAH	Mean, (ng/cig)^b	RSD, (%)	Mean, (ng/cig)^b	RSD, (%)	Mean, (ng/cig)^b	RSD, (%)	Mean, (%)	RSD, (%)	Published values, (ng/cig) [10]	Published values, (ng/cig) [13]	Published values, (ng/cig) [37]
Naphthalene	385.3	7	367.3	6	479.6	22	73	7	287 ± 36	360.8	NR
Benzo[c]phenanthrene	2.0	35	< 0.5	NA	4.8	5	–^c	–	NR	NR	NR
Benzo[a]anthracene	12.6	5	11.2	23	13.3	3	81	3	13 ± 1.4	14.1	11.8
Chrysene	15.3	10	18.5	12	17.6	10	59	4	17 ± 1.8	16.2	NR
Cyclopenta-[c,d]pyrene	5.0	22	10.7	13	7.0	5	–	–	NR	NR	NR
5-Methylchrysene	0.2	14	< 1.5	NA	0.2	13	–	–	NR	NR	< 0.4
Benzo[b]fluoranthene	5.1	14	5.5	7	5.7	6	79	4	8.3 ± 1.0	5.4	5.09
Benzo[k]fluoranthene	2.2	13	2.4	6	2.9	4	83	3	1.6 ± 0.3	2.2	2.02
Benzo[j]aceanthrylene	0.4	20	< 2.5	NA	0.3	15	66	8	NR	NR	NR
Benzo[a]pyrene	6.7	12	6.2	5	7.3	4	75	3	7.0 ± 0.8	6.6	6.73
Indeno[1,2,3-cd]pyrene	3.1	9	0.7	85	0.4	6	78	5	NR	3.8	2.87
Dibenzo[ah]anthracene	0.4	6	3.2	7	3.1	6	80	4	NR	NR	< 0.97
Dibenzo[a,l]pyrene	0.03	34	< 7.5	NA	< 0.2	NA	–	–	NR	NR	< 0.19
Dibenzo[a,e]pyrene	0.1	32	0.4	16	0.3	8	82	10	NR	NR	0.173
Dibenzo[a,i]pyrene	0.1	79	0.4	32	< 0.2	NA	86	13	NR	NR	< 0.22
Dibenzo[a,h]pyrene	0.1	59	0.4	2	< 0.2	NA	–	–	NR	NR	< 0.23

IS internal standard, NA not applicable, NR not reported, RSD relative standard deviation
^aRecovery calculated from GC–HRMS data
^bn = 6 replicates
^c¹³C mass labelled internal standards were not available

ISSN: 2661-801X