Fig. 4From: The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosaa Representative UPLC-MS single ion monitoring (SIM) chromatograms for isomers of diCQA following PHWE of B. pilosa at 50 °C using 60 % MeOH (A), 40 % MeOH (B), 20 % MeOH (C) and 0 % MeOH (water) (D). b Representative UPLC-MS single ion monitoring (SIM) chromatograms for chicoric acid and chicoric acid isomer following PHWE of B. pilosa at 50 °C using 60 % MeOH (A), 40 % MeOH (B), 20 % MeOH (C) and 0 % MeOH (water) (D)Back to article page