Table 1 Chromatographic conditions adopted for gradient HPLC separation of reaction components in the enzymatic synthesis of N-lauroyl N-methyl glucamide
From: Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant
Mobile phase | Solvent A = | Water: trifluoroacetic acid (0.05% w/w) | |
Solvent B = | Methanol | ||
Gradient | Time (min) | % Solvent A | % Solvent B |
0 | 25 | 75 | |
5 | 25 | 75 | |
10 | 5 | 95 | |
10 | 5 | 95 | |
5 | 25 | 75 | |
10 | 25 | 75 | |
Flow rate | 1 ml/min | ||
Column | LiChrospher® 100 RP-18 (5 μm) (LiChroCART® 125-4 HPLC cartridge) Merck, Darmstadt, Germany | ||
Column temperature | 40°C | ||
Detection | ELSD (Alltech 3300, Alltech Associates, USA) | ||
Injection volume | 5 ul | ||
ELSD settings | |||
Temperature | 38°C | ||
Gas flow (air) | 1.3 L/min | ||
Gain | 1 | ||
Run time | 40 min | ||