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Table 4 Process variables and their levels used in CCD

From: Ultrasound-assisted lipase-catalyzed synthesis of D-isoascorbyl palmitate: process optimization and Kinetic evaluation

  

Levels

Independent variables

Coded symbols

-2 -1 0 1 2

Temperature (°C)

A(X1)

30

40

50

60

70

Molar ratio (D-isoascorbic: palmitic acid)

B(X2)

1:1

1:2

1:3

1:4

1:5

Enzyme load (w/w)

C(X3)

0.5

5

9.5

14

18.5

Ultrasonic power (W)

D(X4)

60

90

120

150

180