D-isoascorbyl palmitate: lipase-catalyzed synthesis, structural characterization and process optimization using response surface methodology

Table 1 Influence of the lipase source on the synthesis of D- isoascorbyl palmitate

Lipase	Origin	Immobilized matrixe	Effective temperature (°C)	Specific activity	Water content	Conversion rate (%)^a
Novozyme 435	Candida antarctica	Macroporous acrylic resin	40-60	10,000PLU/g^b	1-2%	41.30 ± 2.6
Lipozyme TLIM	Thermomyces lanuginosus	Silica granulation	55-70	250IUN/g^c	5%	4.30 ± 1.9
Lipozyme RMIM	Rhizomucor miehei	Anionic exchange resin	30-70	5-6BAUN/g^d	2-3%	15.20 ± 3.5
LVK-H100	Aspergillus nige		15-45	20,000U/g		0
LBK-B400	Aspergillus nige		25-65	30,000U/g		0

a: Reaction conditions: D-Isoascorbic 2.5 mmol palmitic acid 10 mmol (Molar ratio was 1:4), lipase load: 15% (weight % of substrates), temperature 50°C, tert-amyl alcohol 20 mL, 50 g/L molecular sieve 4 Å and 200 rpm speed for 24 h.
b: PLU is based on a reaction between propyl alcohol and lauric acid.
c: Interesterification Unit ( IUN) is international unit, based on tributyrin assay.
d: Batch Acidolsis Units Novo (BAUN) is based on a reactionbetween high oleic sunflower oil and decanoic acid at 70- 80°C for 60 min.

ISSN: 2661-801X