Starting trehalose (percent wv-1) | percent trehalose precipitated | Enzyme loadinga (weight percentage) | Initial rateb (nmoles mg-1min-1) | Visual morphology of enzyme precipitate | Precipitation of trehalose in propanol (control) |
---|---|---|---|---|---|
0 | 0 | - | 16.6 | Powder (EPRP) | No precipitation |
2 | 65 | 38.9 | 40.0 | Powder (EPRP) | No precipitation |
5 | 90 | 15.5 | 45.2 | Powder (EPRP) | No precipitation |
10 | 94 | 7.5 | 63.2 | Powder-crystalline | No precipitation |
15 | 88 | 5.9 | 120.0 | Crystalline | Precipitation |
20 | 85 | 4.6 | 125.1 | Crystalline | Precipitation |
25 | 78 | 4.0 | 143.2 | Crystalline | Precipitation |
30 | 76 | 3.5 | 150.5 | Crystalline | Precipitation |
35 | 72 | 3.1 | 152.0 | Crystalline | Precipitation |
40 | 70 | 2.8 | 151.2 | Crystalline | Precipitation |
45 | 65 | 2.3 | 96.4 | Crystalline | Precipitation |
Saturated trehalose solution | 60 | 1.5 | 87.4 | Crystalline | Precipitation |
PCMC (K2SO4) | - | 5.8 | 132.5 | Crystalline | Precipitation |