Transition metal complexes of an isatinic quinolyl hydrazone

Background The importance of the isatinic quinolyl hydrazones arises from incorporating the quinoline ring with the indole ring in the same compound. Quinoline ring has therapeutic and biological activities. On the other hand, isatin (1H-indole-2,3-dione) and its derivatives exhibit a wide range of biological activities. Also, the indole ring occurs in Jasmine flowers and Orange blossoms. Recently, the physiological and biological activities of quinolyl hydrazones arise from their tendency to form metal chelates with transition metal ions. In this context, we have reported to isolate, characterize and study the biological activity of some transition metal complexes of an isatinic quinolyl hydrazone; 3-[2-(4-methyl quinolin-2-yl)hydrazono] indolin-2-one. Results Mono- and binuclear as well as dimeric chelates were obtained from the reaction of a new isatinic quinolyl hydrazone with Fe(III), Co(II), Ni(II), Cu(II), VO(II) and Pd(II) ions. The ligand showed a variety of modes of bonding viz. (NNO)2-, (NO)- and (NO) per each metal ion supporting its ambidentate and flexidentate characters. The mode of bonding and basicity of the ligand depend mainly on the type of the metal cation and its counter anion. All the obtained Pd(II)- complexes have the preferable square planar geometry (D4h- symmetry) and depend mainly on the mole ratio (M:L). Conclusion The effect of the type of the metal ion for the same anion (Cl-) is obvious from either structural diversity of the isolated complexes (Oh, Td and D4h) or the various modes of bonding. The isatinic hydrazone uses its lactim form in all complexes (Cl-) except complex 5 (SO42-) in which it uses its lactam form. The obtained Pd(II)- complexes (dimeric, mono- and binuclear) are affected by the mole ratio (M:L) and have the square planar (D4h) geometry. Also, the antimicrobial activity is highly influenced by the nature of the metal ion and the order for S. aureus bacteria is as follows: Nickel(II) > Vanadyl(II) > Cobalt(II) > Copper(II) ≈ Palladium(II) >> Iron(III).


Characterization of the hydrazone
The results of elemental analysis of the investigated hydrazone (H 2 L; Scheme 1) are in good agreement with the proposed molecular formula; C 18  The IR spectrum showed very strong bands at 3333, 1684 and 1633 cm -1 assignable to ν(NH), ν(C = O) and ν(C = N), respectively, confirming the lactam nature of H 2 L. Also, the 1 H NMR spectral data of the ligand in d 6 -DMSO relative to TMS ( Figure 1) provide a further support of the lactam nature of the hydrazone. The mass spectrum of the ligand showed the M + peak at m/z = 302 and the base peak at m/z = 273 confirming its formula weight. Furthermore, the mass fragmentation pattern (Scheme 2) supported the suggested structure of the ligand.
Effect of solvent on the spectra of the hydrazone In this study, the electronic absorption spectra of the hydrazone (2 × 10 -5 M) were recorded in solvents of various polarities (Table 1 and Figure 2). The hydrazone displays mainly three bands, the shortest UV-bands (λ 1 = 205-275 and λ 2 = 384-401 nm ) are best ascribed to π-π* transitions of the aromatic system and they are solvent sensitive bands whereas, the longest UV-band (λ 3 = 468-476 nm) is a strong broad band reflecting its charge transfer (CT) nature [9,10]. The latter band impacts the ligand its red color. Most bands suffered a red shift on increasing the solvent polarity. Also, the less intense n-π* transitions of the C = O and C = N are hidden under the more intense CT bands, especially in polar solvents. Correlation of the band shift ( ν) with the solvent parameters [8,[11][12][13]  dielectric constant), Kosower's (Z), Dimroth-Reichardt's (E T ), Kamlet-Taft's (α, β, π*) or Gutmann's donoracceptor numbers (DN, AN) is not satisfactory indicating that ν is affected by more than one parameter e.g. polarity, donor-acceptor and acid-base properties of the solvents as well as solute-solvent interactions and H-bonding.

Characterization of the isatinic complexes
The isatinic hydrazone was allowed to react with copper (II), cobalt(II), nickel(II), and iron(III) chlorides, as well as vanadyl (II) sulfate monohydrate at the mole ratio; 1:1 (Scheme 3). Also, in an attempt for investigating the effect of the mole ratio on the products, the isatinic hydrazone was allowed to react with palladium(II)-chloride at the mole ratios 1:1, 1:2 and 2:1 (M:L). These reactions afforded dimeric, mono-and binuclear complexes (Table 2 and Scheme 4). The obtained Pd(II)-complexes have the preferable square planar (D 4h ) geometry [14]. In case of the ratio 1:2 (M:L), a base catalyzed dimerization occurs (Scheme 4; complex 7 indolin-2-one [10]. This is due to the presence of the Megroup in the 8-position in close proximity to the heterocyclic N-atom of the quinoline ring [6]. Hence, the degree of basicity and deprotonation of the hydrazone as well as the mode of bonding are highly affected by the position of the Me-group. The dimeric complexes (2 and 7) have the trans arrangement and are formed via lactim bridging, whereas the binuclear complex (8) is formed via chloride bridging. Characterization of the obtained complexes was achieved via elemental analyses, magnetic and conductivity measurements as well as spectral studies.

IR spectra of the complexes
Most complexes showed a broad band in the range 3463-3203 cm -1 due to ν(OH) of the associated water or ethanol molecules. The band at 1633 cm -1 assignable to ν(C = N) in the free ligand was shifted to higher or lower values indicating its participation in the chelation with πelectron delocalization. In all complexes, the band located at 1684 cm -1 due to ν(C = O) of the free ligand disappeared indicating the participation of the lactim form in the chelation. An exception is complex 5 in which this band is shifted to lower wave number; 1670 cm -1 . The variable intensity bands of the quinoline ring of the free hydrazone at 1554,1482 and 1455 cm -1 are greatly altered as a result of the complexation. The free sulfate ion belongs to the high symmetry T d point group. Of the four fundamentals, only υ 3 and υ 4 are IR active; bands at~1105 and~615 cm -1 , assignable to υ 3 stretching [υ(SO)] and υ 4 bending [δ(OSO)] modes, respectively [15,16]. The υ 1 stretching [υ(SO)] and υ 2 bending [δ(OSO)] fundamentals are not IRactive. However, the coordination of SO 4 2to metal ions in a bidentate fashion decreases the symmetry of the group and the υ 3 and υ 4 modes may be split [15][16][17]. For the sulfato complex (5) in this study, the chelating bidentate nature of the SO 4 2group is indicated by the  strong band at 1100 cm -1 ; ν 3 (S-O) as well as a medium band at 657 cm -1 ; ν 4 [δ(OSO)] characteristic for the tetrahedral (T d ) point group. These spectral features suggest a low symmetry for the sulfato ligand in the complex.

Magneto chemistry
The magnetic properties of the complexes provide valuable information for distinguishing their stereochemistry. Iron (III) has a 3d 5 configuration and its complexes may be either high-spin (S = 5/2), intermediate-spin (S = 3/2) or low-spin (S = 1/2), however, the majority of iron(III)-complexes are high-spin. The effective magnetic moment (μ eff ) of the iron(III)-complex (1) has the value 6.13 B.M. (Table 3) which is consistent with five unpaired electrons suggesting an sp 3 d 2 hybridization, and falls within the range reported for high spin O hgeometry [18]. The copper(II)-complex (4), 3d 9 -system and oxovanadium (IV) complex (5); 3d 1 -system exhibit μ eff values of 1.83 and 1.91 B.M. (Table 3) indicating the  presence of one unpaired electron. In addition, the cobalt(II)-complex (2); 3d 7 -system showed an effective magnetic moment of 1.46 B.M. confirming its square planar geometry which is related also to one unpaired electron. This value confirms its dimeric nature (μ eff < 1.73 B.M.; spin only value in absence of a ligand field).
In general, square planar complexes rather than tetrahedral complexes usually arise from strong field ligands, the stronger the ligand, the more favored is the trans isomer. On the other hand, the nickel(II)-complex (3); 3d 8 -system showed μ eff value of 2.96 B.M. which is consistent with two unpaired electrons confirming its tetrahedral (T d ) geometry (all square planar nickel(II)complexes are diamagnetic). Conversely, room temperature magnetic moment data (Table 3) showed the diamagnetism of the palladium(II)-complexes (6)(7)(8) in consistency with their square planar geometry; a general phenomenon for palladium(II)-complexes [14]. The ligands tending to give a strong field and to cause spin pairing give 4-or 5-rather than 6-coordinate complexes. The structure of the dimeric complex 7 has some interesting features; the Pd(II)-ions are at a center of a square plane of the chromophore N 2 O 2 . These planes are stacked one above the other in the crystal, alternate molecules, being twisted through 90°, so the Pd(II) ions form chains with a Pd-Pd bond. Thus, the diamagnetic Pd(II)-complexes may be regarded as containing either square planar or tetragonally coordinated palladium with metal-metal bonding [19].

Mass, ESR and electronic spectra
The  (Figure 3 and Table 3). They showed new intense broad bands in the range 504-518 nm confirming their charge transfer (CT) nature. Therefore, the color of all complexes is dominated by a CT transition which obscured the weak d-d transitions occurring in the same region; a phenomenon encountered with isatinic complexes [20,21]. The X-band ESR spectrum of powdered sample of [Cu(L)(H 2 O)] (4) at room temperature exhibited one broad signal with g = 2.17. The shape of the spectrum is consistent with the square-planar geometry around Cu(II) center in the complex [22]. Also, the X-band ESR spectrum of [VO(H 2 L)(SO 4 ) (H 2 O)]; 5 gave a broad homogeneous signal centered on g = 2.12 without resolved hyperfine structure; 51 V (I = 7/2, S = 1/2). The absence of vanadium's hyperfine coupling is common in the solid state and is attributed to the simultaneous flipping of neighboring electron spin or strong exchange interactions, which average out the interaction with the nuclei [23,24]. However the gvalues show positive deviation from the g = 2.0023 (the value of free electron), indicating a covalent character in the M-L bonding.

Antimicrobial activity
The antimicrobial activity of the ligand and its metal complexes were summarized in Table 4 and represented graphically in Figure 4. Inspection of the data revealed the following: a) The ligand lacks the antimicrobial activity. b) Also, complexes 1, 2 and 4 lack the antimicrobial activity towards Gram negative bacteria. c)

Superiority of the binuclear Pd(II)-complex (8) against
S. pyogenes bacteria [25,26]. d) The antimicrobial activity is highly influenced by the nature of the metal ion ( Figure 4) and the order for Gram-positive bacteria (S. aureus) is as follows: Nickel(II) > Vanadyl(II) > Cobalt(II) > Copper(II) ≈ Palladium(II) >> Iron(III). In this context, the activity of the isatinic complexes is related to the chelated indole ring [3]. The chelation increases the lipophilic character of the metal chelate and favors its permeation through the lipoid layer of the bacterial membranes [27,28]. e) Also, the degree of inhibition is influenced by the type of the coordinated anion as well as the concentration. f) Finally, all the investigated complexes exhibited low to moderate activities against the studied organisms relative to the standard reference (control). In conclusion, the isatinic complexes gave a fair inhibitor effect on growth of the microorganisms and were more potent as bacteriostatic agents.

Materials
The chemicals used in this investigation were of the highest purity available (Merck, BDH, Aldrich and Fluka). They included copper(II), cobalt(II), nickel(II), palladium(II) and iron(III) chlorides, as well as vanadyl sulfate monohydrate. Also, they included aniline, ethyl acetoacetate, phosphorus oxychloride, hydrazine hydrate and isatin. Organic solvents were reagent grade chemicals and were used without further purification.

Measurements
Microanalyses were carried out on a Perkin-Elmer 2400 CHN elemental analyzer. Analyses of metal ions followed dissolution of the solid complex in hot concentrated nitric acid, HNO 3 , then diluting with doubly distilled water and filtration. The resultant solution was neutralized with ammonia and the metal ions were then titrated against EDTA. Electronic spectra were recorded on a Jasco V-550 UV/VIS spectrophotometer. IR spectra were recorded on a Bruker Vector 22 spectrometer using KBr pellets. ESR spectra were recorded on a Bruker Elexsys, E 500 operated at X-band frequency. Mass spectra were recorded either at 70 eV on a gas chromatographic GCMSQP 1000-EX Shimadzu mass spectrometer or Direct Inlet unit (DI-50) of Shimadzu GC / MS -QP5050A. 1 H NMR spectra were recorded as DMSO-d 6 solutions on a Varian Mercury VX-300 NMR spectrometer using TMS as a reference.   Preparation of the isatinic hydrazone [5][6][7][8][9] An ethanolic mixture of 2-hydrazinyl-4-methyl quinoline (0.01mol) and isatin (0.012 mol) was refluxed for 15 min. The formed scarlet red compound was filtered off, washed with ethanol and crystallized from DMF; Yield: 77% and m.p 302°C.

Preparation of the metal complexes
Ethanolic solutions of the metal salt and the ligand were mixed in the mole ratio 1:1 (M:L) and refluxed for 2-4 hours depending on the nature of the isolated complexes (1)(2)(3)(4)(5)(6). Also, the mole ratio 1:2 and 2:1 (M:L) was tried in case of PdCl 2 (complexes 7 and 8). The resulting precipitates were filtered off, washed with ethanol then ether and finally air-dried. The results of elemental analysis and % yield are shown in Table 2. All the isolated complexes are stable at room temperature, nonhygroscopic and insoluble in water and alcohols and soluble in DMSO and DMF. The melting points of the complexes were above 300°C. The molar conductance of milli-molar DMF solutions indicates a non-electrolytic nature for all complexes (Table 3).

Antimicrobial activity
The standardized disc-agar diffusion method [29] was followed to determine the activity of the synthesized compounds against the sensitive organisms Staphylococcus aureus (ATCC 25923) and Streptococcus pyogenes (ATCC19615) as Gram-positive bacteria, Pseudomonas fluorescens (S 97) and Pseudomonas phaseolicola (GSPB 2828) as Gram-negative bacteria. The antibiotics chloramphencol and Cephalothin were used as standard reference in case of Gram-negative and Gram-positive bacteria, respectively. The tested compounds were dissolved in dimethyl formamide (DMF) which have no inhibition activity to get concentrations of 2 mg / mL and 1 mg / mL. The test was performed on medium potato dextrose agar (PDA) which contain infusion of 200 g potatoes, 6 g dextrose and 15 g agar [29] Uniform size filter paper disks (3 disks per compound) were impregnated by equal volume (10 µL) from the specific concentration of dissolved tested compounds and carefully placed on inoculated agar surface. After incubation for 36 h at 27°C, inhibition of the organisms which evidenced by clear zone surround each disk was measured and used to calculate mean of inhibition zones.

Conclusion and comments
The effect of the type of the metal ion for the same anion (Cl -) is obvious from either the geometry of the isolated complexes (O h , T d and D 4h ) or the various modes of bonding. The isatinic hydrazone uses its lactim form in all complexes (Cl -) except complex 5 with doubly charged SO 4 2anion in which it uses its lactam form. For the tetra-coordinated (tetrahedral or square planar) Co (II) and Ni(II)-complexes, their geometries are well defined by the magnetic measurements. Tetrahedral Co (II)-complexes have three unpaired electrons and square planar only one. Similarly, tetrahedral Ni(II)-complexes have two unpaired electrons and square planar are diamagnetic. Therefore, the obtained μ eff values of 1.46 and 2.96 B.M. (Table 3) for Co(II) and Ni(II)-complexes (2,3) support the dimeric square planar of the former and a tetrahedral environment of the later. In case of Palladium (II)-complexes (6)(7)(8), the isatinic hydrazone produces ligand field strong enough to cause spin pairing. On the other hand, the antimicrobial activity is highly influenced by the nature of the metal ion and the order for S. aureus bacteria is as follows: Nickel(II) > Vanadyl(II) > Cobalt