- Research article
- Open Access
Synthesis and fungicidal activity of pyrazole derivatives containing 1,2,3,4-tetrahydroquinoline
© The Author(s) 2016
- Received: 30 January 2016
- Accepted: 20 June 2016
- Published: 4 July 2016
Take-all of wheat, caused by the soil-borne fungus Gaeumannomyces graminis var. tritici, is one of the most important and widespread root diseases. Given that take-all is still hard to control, it is necessary to develop new effective agrochemicals. Pyrazole derivatives have been often reported for their favorable bioactivities. In order to discover compounds with high fungicidal activity and simple structures, 1,2,3,4-tetrahydroquinoline, a biologically active group of natural products, was introduced to pyrazole structure. A series of pyrazole derivatives containing 1,2,3,4-tetrahydroquinoline were synthesized, and their fungicidal activities were evaluated.
The bioassay results demonstrated that the title compounds displayed obvious fungicidal activities at a concentration of 50 μg/mL, especially against V. mali, S. sclerotiorum and G. graminis var. tritici. The inhibition rates of compounds 10d, 10e, 10h, 10i and 10j against G. graminis var. tritici were all above 90 %. Even at a lower concentration of 16.7 μg/mL, compounds 10d and 10e exhibited satisfied activities of 100 % and 94.0 %, respectively. It is comparable to that of the positive control pyraclostrobin with 100 % inhibition rate.
- Fungicidal activity
- Wheat take-all
Wheat (Triticum aestivum) is one of the most important crops in the world. Take-all of wheat, caused by the soil-borne fungus Gaeumannomyces graminis var. tritici, is one of the most serious and widespread root diseases [1, 2]. The pathogen infects the roots of susceptible plants, resulting in black necrotic, plant stunting, white heads, and etc. [3, 4]. It reduces the grain yield from 20 % up to 50 %. Unfortunately, the control of take-all is still a huge problem. And the application of agrochemicals is currently the most effective method . However, existing chemical control agents, such as silthiopham, were not financially affordable for the control of wheat take-all . Hence, it is necessary to develop effective and inexpensive agents to replace the conventional agrochemicals.
In recent years, pyrazole derivatives have attracted tremendous attention owing to their excellent bioactivities [20–22]. Pyraclostrobin (Fig. 1) discovered by BASF is a commercial fungicide containing pyrazole structure. It came to the market in 2002. Given its wide fungicidal spectrum, pyraclostrobin had achieved a total sale of $800 million in 2012, ranked the second in the world. . Besides, pyrazole derivatives were also reported to possess insecticidal activities [24, 25], herbicidal activities , and anticancer activities [27, 28].
It is an effective method to develop new green agrochemicals by introducing active groups of natural products to known active sub-structures. As above mentioned, THQ is an important active group of natural products. In order to find highly biologically active lead compounds with simple structures, THQ was introduced to the known active sub-substructure of pyrazole compounds using intermediate derivatization methods (IDM) . A series of pyrazole derivatives containing 1,2,3,4-tetrahydroquinoline were synthesized, and their activities were evaluated in this study. Biological assays revealed that some compounds exhibited good fungicidal activities. Especially, they displayed excellent activities against G. graminis var. tritici.
The structures of all the title compounds were confirmed by 1H NMR, 13C NMR, IR spectra and HRMS or elemental analysis and the relevant data could be found in the Additional file 1. Compound 10a was taken as an example to analyze the 1H NMR spectra data. Four protons of the benzene ring were observed at δ 7.18–6.87. A single peak at δ 5.76 was due to the proton at the 4-position of the parazole ring. Two protons at the 2-position of THQ were observed at δ 3.90 with J = 6.5 Hz as a triple peak, and the other triple peak at δ 2.82 with J = 6.6 Hz was due to the protons at the 4-position of THQ. Two protons at the 3-position of THQ was showed at δ 2.03 with J = 6.6 Hz as pentaploid peaks. The chemical shifts as single peaks were observed at δ 3.87 and 2.15 due to the protons of N-CH3 and CH3 at the 3-position of the parazole ring respectively.
Fungicidal activities of title compounds against seven kinds of pathogenic fungi
Fungicidal activity (%)/50 μg/mL
G. g. t
Primary structure activity relationships (SAR) revealed that the substituents played an important role in fungicidal activities. (1) When substituent R1 was methyl, compounds with R2 as (substituted) phenyl exhibited better activities than those with R2 as alkyl (10d, 10e, 10f > 10a, 10b, 10c). (2) When R1 was phenyl, the fungicidal activities increased with the increase of the carbon number in the alkyl chain of the R2 moiety (10g < 10h < 10i ≈ 10j). However, fungicidal activities decreased dramatically when R1 and R2 were both phenyl (10k). (3) It was not beneficial to increase their fungicidal activities when R1 was substituted pyridyl (10o and 10p).
In particular, compounds 10d (R1 = Me, R2 = Ph), 10e (R1 = Me, R2 = 4-OMePh), 10i (R1 = Ph, R2 = n-Pr) and 10j (R1 = Ph, R2 = i-Pr) exhibited good activities against V. mali, S. sclerotiorum and G. graminis var. tritici with inhibition rates of more than 80 %. Compounds 10d and 10e showed comparable activities against V. mali and G. graminis var. tritici with the commercial fungicide pyraclostrobin.
Dosage-dependent in vitro fungicidal activities of 10d, 10e, 10h, 10i, 10j and pyraclostrobin against G. graminis var. tritici
Inhibition rate (%) at different concentrations (μg/mL)
Melting points of all compounds were determined on an X-4 binocular microscope (Fukai Instrument Co., Beijing, China) without calibration. NMR spectra were acquired with a Bruker 300 MHz spectrometer with CDCl3 as the solvent and TMS as the internal standard. Chemical shifts are reported in δ (parts per million) values. High resolution mass spectrometry (HRMS) data were obtained on an FTICR-MS Varian 7.0T FTICR-MS instrument. Elemental analysis was carried out on a Vario EL III elemental analyzer. All the reagents were obtained commercially and used without further purification. Column chromatography purification was carried out by using silica gel. The synthesis of intermediates and title compounds can be found in the Additional file 1.
Antifungal biological assay
All the target compounds have been evaluated for their in vitro fungicidal activities against seven pathogenic fungi, using mycelium growth rate method according to the literature [35, 36]. Fungi tested in this article included Pythium aphanidermatum, Rhizoctonia solani, Valsa mali, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium moniliforme and Gaeumannomyces graminis var. tritici. Dimethyl sulfoxide (DMSO) in sterile distilled water served as the control. Pyraclostrobin (Fig. 1) containing pyrazole structure (Fig. 1) as the commercial fungicide, was assessed under the same conditions as a positive control. In the preparation, every compound (10 mg) was weighted accurately and dissolved in 1 mL DMSO, and then it was mixed with 200 mL potato dextrose agar (PDA). As a consequence, they were tested at a concentration of 50 μg/mL. In order to get new mycelium for antifungal assay, all fungal species were incubated in PDA at 25 ± 1 °C for 1–7 days vary from different fungi. Mycelia dishes were cut with a 5 mm in diameter hole punch from the prepared edge of culture medium. One of them was picked up with a sterilized inoculation needle, and then inoculated in the center of the PDA plate aseptically. Every treatment repeated three times, and they were incubated at 25 ± 1 °C for 1–7 days vary from different fungi. All the above was completed in a bioclean environment. The hypha diameter was measured by a ruler, and the data were statistically analyzed. The inhibition rate of the title compounds on the fungi was calculated by the following formula:
I (%) = [(C − T)/(C − 5)] × 100, where I is the inhibition rate, C represents the diameter (mm) of fungal growth on untreated PDA, and T represents the diameter (mm) of fungi on treated PDA.
In summary, a series of pyrazole derivatives containing 1,2,3,4-tetrahydroquinoline were synthesized and their structures were confirmed by 1H NMR, 13C NMR, IR and HRMS or elemental analysis. The crystal structure of compound 10g was determined by X-ray diffraction. Bioassay results indicated that all the title compounds exhibited good fungicidal activities. And the substituents played an important role in fungicidal activities. In particular, compounds 10d and 10e with simple structures showed comparable activities against G. graminis var. tritici to the commercial fungicide pyraclostrobin even at the concentration 16.7 μg/mL. These two compounds could be valuable leads for further studies.
The current study is an outcome of constructive discussion with XLY and YL; PL carried out the synthesis, characterization and antifungal bioassay experiments and involved in the drafting of the manuscript. XLL involved in the antifungal bioassay; XBZ and YX partly involved in the synthesis of title compounds; GFX and XHZ partly involved in the synthesis of intermediates. All authors read and approved the final manuscript.
This work was financially supported by the National Natural Science Foundation of China (No. 21272266).
The authors declare that they have no competing interests.
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