- Research article
- Open Access
Heavy metal ions in wines: meta-analysis of target hazard quotients reveal health risks
© Naughton et al 2008
- Received: 24 July 2008
- Accepted: 30 October 2008
- Published: 30 October 2008
Metal ions such as iron and copper are among the key nutrients that must be provided by dietary sources. Numerous foodstuffs have been evaluated for their contributions to the recommended daily allowance both to guide for satisfactory intake and also to prevent over exposure. In the case of heavy metal ions, the focus is often on exposure to potentially toxic levels of ions such as lead and mercury. The aim of this study is to determine target hazard quotients (THQ) from literature reports giving empirical levels of metal ions in table wines using the reference upper safe limit value. Contributions to the THQ value were calculated for seven metal ions along with total values for each wine.
The THQ values were determined as ranges from previously reported ranges of metal ion concentrations and were frequently concerningly high. Apart from the wines selected from Italy, Brazil and Argentina, all other wines exhibited THQ values significantly greater than one indicating levels of risk. The levels of vanadium, copper and manganese had the highest impact on THQ measures. Typical potential maximum THQ values ranged from 50 to 200 with Hungarian and Slovakian wines reaching 300. THQ values for a sample of red and white wines were high for both having values ranging from 30 to 80 for females based on a 250 mL glass per day.
The THQ values calculated are concerning in that they are mainly above the safe level of THQ<1. It is notable that in the absence of upper safe limits, THQ values cannot be calculated for most metal ions, suggesting that further unaccountable risks are associated with intake of these wines.
- White Wine
- Target Hazard Quotient
- Oral Reference Dose
- French Wine
- Italian Wine
As for many food components, the intake of metal ions can be a double edged sword. The requirement for ingestion of trace metals such as Fe and Cu ions to maintain normal body functions such as the synthesis of metalloproteins is well established. However, cases of excess intake of trace metal ions are credited with pathological events such as the deposition of iron oxides in Parkinson's disease . In addition to aiding neurological depositions, these redox active metals ions have been credited with enhancing oxidative damage, a key component of chronic inflammatory disease  and a suggested initiator of cancer . As inflammation is a characteristic feature of a wide range of diseases, further potential pathological roles for metal ions are emerging as exemplified by premature ageing .
For the maintenance of health, a great deal of preventative measures are in place to avoid ingestion of potentially toxic metal ions. From monitoring endogenous levels of metal ions in foods and drinks to detecting contamination during food preparation, European countries spend significant resources to avoid metal intake by the general population [5–7].
From a therapeutic viewpoint, considerable research and development efforts are being exerted to decorporate metal ions from the body. Since the use of As in World War I, researchers have advanced methods to decorporate toxic metals ions [8, 9]. More recently efforts have moved to erradicate neurological deposits and reverse redox-active metal ion contributions to oxidative stress . The latter approach has a focus on chelators that reverse the potential detrimental effects by generating anti-oxidant enzyme mimetics upon chelating the labile redox-active metal ion. Intriguingly, some very good candidates for anti-oxidant pro-drug chelators are common food constituents such as catechins [10, 11].
Target hazard quotients (THQ) were developed by the Environmental Protection Agency (EPA) in the US for the estimation of potential health risks associated with long term exposure to chemical pollutants . The THQ is a ratio between the measured concentration and the oral reference dose, weighted by the length and frequency of exposure, amount ingested and body weight. The THQ value is a dimensionless index of risk associated with long term exposure to chemicals based upon reference upper safe limits. A limited number of THQ investigations have been reported in foodstuffs with the focus being on estimating health risks associated with exposure to heavy metals found in seafoods, and in one case breast milk [12–18]. Calculations of THQ values for seafoods are apposite as many species accumulate heavy metals and other pollutants in their tissues. Many of the reported THQ values calculated from metal contaminants in seafood range from a safe level (<1) to a level of concern (typically THQ >1 to <5) with a small number being above 10. It should be noted that THQ values are additive, not multiplicative, thus a THQ value of 20 is larger but not ten-fold greater than a THQ = 2.
The authors have recently reported the first application of THQ estimations to common beverages . THQ values for daily ingestion of 250 mL of apple juice, stout and red wine were all above the safe value of 1. The THQ values for red wine were especially high at 126.2 for males and 157.22 for females (with gender variations owing to the differences in average weight and lifespan). In this study, individual THQ values were calculated for seven metal ions for which oral reference doses exist (V, Cr, Mn, Ni, Cu, Zn and Pb). It is notable that these relatively high THQ values were determined using only seven metal ions out of some thirty measured. It is conceivable that other metal constituents will contribute to the total THQ values when their upper safe limits are established.
In addition to their roles in health and disease, dietary metal ions have been the focus of discussions on the mechanism of ageing. Redox-active metal ions such as Cu(I)/(II) and Fe(II)/(III) are especially implicated in the free radical theory of ageing as they are credited with enhancing oxidative stress [2, 4, 20]. However, beyond radicals, metal ions can disrupt normal cell and tissue function through multiple pathways including interactions with proteins and other biomolecules and disruption of membrane potentials . The aim of this study is to determine target hazard quotients (THQ) from literature reports which give empirical levels of metal ions in table wines.
As anticipated from the previous study , V has a large impact on the overall THQ values with values ranging up to >360 (for females) for some Hungarian and Slovakian wines. THQ values based on V for French wines range from below 60 to over 200 (for females) with Portuguese wines having a range of 100 to >140 (for females). Wines from Germany and Argentina also have a spread of THQ values based levels of V ions which are concerning. For Cu ions large variations in the contribution to overall THQ values are observed for wines from Spain, Jordan and Macedonia with upper limits of circa 70, 60 and 40 respectively for females. Lower ranges are observed for the Cu ion-based THQ value for the other seven countries with all except Argentina being of concern (THQ>1).
It should be noted that the THQ estimation is a risk assessment designed to avoid underestimation of the risk. Thus, it incorporates several assumptions such as ingested quantities of metal ions correspond to the quantities that are absorbed . On the contrary, many metal ions have been shown to be hazardous but do not yet have an oral reference dose. In addition, bolus dosing (e.g. binge drinking) and cross effects with other potential toxins (e.g. alcohol) are not accounted for, nor are the effects on the elderly or on the young considered. In the same vein THQ values do not reflect genetic predispositions to disease or people with clinical or sub-clinical conditions.
The results from this study also question a popular belief about the health-giving properties of red wine: that drinking red wine daily protects you from heart attacks is often related to levels of anti-oxidants. However the finding of hazardous levels of metal ions which can be pro-oxidants leads to a major question mark over the protective benefits of red wine.
Previous reports of levels of metals ion wines were selected to reflect both red and white wines from a variety of countries. Reports were selected if they included levels of key metal ions for which THQ values can be calculated (owing to the existence of oral reference doses). In addition for a select representative sample THQ values were determined from levels of metal ions in red versus white wines from the same countries.
To assess the level of concern arising from the metal concentrations, THQ values were calculated for the minimum and maximum levels of metals, separately for males and females, based upon length of exposure set to 17,155 days for males and for females based on the average life expectancy of 81.9 and 84.7, respectively from 18 years of age ; and the mean weight (83.11 and 69.81 kg respectively)  for one large glass of wine (250 mL) consumed daily. The THQ values for selected metals were calculated using the method described previously  with the following oral reference doses in mg/kg/d [2, 13]: V (1.0 × 10-3), Cr (1.5), Mn (1.4 × 10-1), Ni (2.0 × 10-2), Cu (4.0 × 10-2), Zn (3.0 × 10-1) and Pb (1.5). For the oral reference dose we used the tolerable upper intake level (UL) [26, 27], which is the highest average daily intake level without the risk of adverse health effects. Intake above the UL could be hazardous to health to almost all individuals in the general population.
Relatively high levels of potentially hazardous metal ions are frequently found in both red and white wines originating from various countries. For consumption of 250 mL daily, these wines give very high THQ values and may present detrimental health concerns through a lifetime based upon the metal content alone. Further research is warranted in this area in the interests of public health to determine the mechanisms of metal inclusion/retention during wine production. These studies should include the influence of grape variety, soil type, geographical region, insecticides, containment vessels and seasonal variations. In addition, levels of metal ions should appear on wine labels along with the introduction of further steps to remove key hazardous metal ions during wine production.
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